Spatio-temporal coordination at the maternal-fetal interface promotes trophoblast invasion and vascular remodeling in the first half of human pregnancy

Greenbaum S, Averbukh I,  Soon E,  Rizzuto G, Baranski A, Greenwald N, Bosse M, Jaswa EG, Khair Z, Kwok S, Warshawsky S, Miller G, Schwartz M, Graf W, Van Valen D, Keren L, Hollmann T,  van de Rijn M, Angelo M​

Robust phenotyping of highly multiplexed tissue imaging data using pixel-level clustering

Liu CC, Greenwald NF, Kong A, McCaffrey EF, Leow KX, Mrdjen D, Angelo M

Combined protein and nucleic acid imaging reveals virus-dependent B cell and macrophage immunosuppression of tissue microenvironments

Jiang S, Chan CN, Rovira-Clavé X, Chen H, Bai Y, Zhu B, McCaffrey E, Greenwald NF, Liu C, Barlow GL, Weirather JL, Oliveria JP, Nakayama T, Lee IT, Matter MS, Carlisle AE, Philips D, Vazquez G, Mukherjee N, Busman-Sahay K, Nekorchuk M, Terry M, Younger S, Bosse M, Demeter J, Rodig SJ, Tzankov A, Goltsev Y, McIlwain DR, Angelo M, Estes JD, Nolan GP

Immunity, June 2022

Reproducible, high-dimensional imaging in archival human tissue by multiplexed ion beam imaging by time-of-flight (MIBI-TOF)

Liu CC, Bosse M, Kong A, Kagel A, Kinders R, Hewitt SM, Varma S, van de Rijn M, Nowak SH, Bendall SC, Angelo M

Laboratory Investigation, March 2022

MITI minimum information guidelines for highly multiplexed tissue images

Schapiro D, Yapp C, Sokolov A, Reynolds SM, Chen YA, Sudar D, Xie Y, Muhlich J, Arias-Camison R, Arena S, Taylor AJ, Nikolov M, Tyler M, Lin JR, Burlingame EA, Human Tumor Atlas Network, Chang YH, Farhi SL, Thorsson V, Venkatamohan N, Drewes JL, Pe’er D, Gutman DA, Herrmann MD, Gehlenborg N, Bankhead P, Roland JT, Herndon JM, Michael MP, Angelo M, Nolan G, Swedlow JR, Schultz N, Merrick DT, Mazzili SA, Cerami E, Rodig SJ, Santagata S, Sorger PK

Nature Methods, March 2022

Multiplexed Ion Beam Imaging: Insights into Pathobiology

Liu CC, McCaffrey EF, Greenwald NF, Soon E, Risom T, Vijayaragavan K, Oliveria JP, Mrdjen D, Bosse M, Tebaykin D, Bendall SC, Angelo M

Annual Review of Pathology: Mechanisms of Disease, January 2022

The immunoregulatory landscape of human tuberculosis granulomas

McCaffrey EF, Donato M, Keren L, Chen Z, Delmastro A, Fitzpatrick M.B, Gupta S, Greenwald NF, Baranski A, Graf W, Kumar R, Bosse M, Fullaway CC, Ramdial PK, Forgó E, Jojic V, Van Valen D, Mehra S, Khader SA, Bendall SC, van de Rijn M, Kalman D, Kaushal D,  Hunter RL, Banaei N, Steyn AJC, Khatri P, Angelo M

Nature Immunology, January 2022

Transition to invasive breast cancer is associated with progressive changes in the structure and composition of tumor stroma

Risom T, Glass DR, Averbukh I, Liu CC, Baranski A, Kagel A, McCaffrey EF, Greenwald NF, Rivero-Gutiérrez B, Strand SH, Varma S, Kong A, Keren L, Srivastava S, Zhu C, Khair Z, Veis DJ, Deschryver K, Vennam S, Maley C, Hwang ES, Marks JR, Bendall SC, Colditz GA, West RB, Angelo M

Cell, January 2022

Single-synapse analyses of Alzheimer's disease implicate pathologic tau, DJ1, CD47, and ApoE

Phongpreecha T, Gajera CR, Liu CC, Vijayaragavan K, Chang AL, Becker M, Fallahzadeh R, Fernandez R, Postupna N, Sherfield E, Tebaykin D, Latimer C, Shively CA, Register TC, Craft S, Montine KS, Fox EJ, Poston KL, Keene CD, Angelo M, Bendall SC, Aghaeepour N, Montine TJ

Science Advances, December 2021

Evaluation of Geuenich et al.: Targeting a crucial bottleneck for analyzing single-cell multiplexed imaging data

Averbukh I, Greenwald NF, Liu CC, Angelo M

Cell Systems, December 2021

Whole-cell segmentation of tissue images with human-level performance using large-scale data annotation and deep learning

Greenwald NF, Miller G, Moen E, Kong A, Kagel A, Dougherty T, Fullaway CC, McIntosh BJ, Leow KX, Schwartz MS, Pavelchek C, Cui S, Camplisson I, Bar-Tal O, Singh J, Fong M, Chaudhry G, AbrahamZ, Moseley J, Warshawsky S, Soon E, Greenbaum S, Risom T, Hollmann T, Bendall SC, Keren L, Graf W, Angelo M, Van Valen D

Nature Biotechnology, November 2021

Spatial mapping of protein composition and tissue organization: a primer for multiplexed antibody-based imaging

Hickey JW, Neumann EK, Radtke AJ, Camarillo JM, Beuschel RT, Albanese AA, McDonough E, Hatler J, Wiblin AE, Fisher J, Croteau J, Small EC, Sood A, Caprioli RM, Angelo M, Nolan GP, Chung K, Hewitt SM, Germain RN, Spraggins JM, Lundberg E, Snyder MP, NL, Saka SK

Nature Methods, November 2021

High-Dimensional Tissue Profiling by Multiplexed Ion Beam Imaging

Elhanani O, Keren L, Angelo M

Single-Cell Protein Analysis, November 2021

Multiplexed imaging reveals an IFN-γ-driven inflammatory state in nivolumab-associated gastritis

Ferrian S, Liu CC, McCaffrey EF, Kumar R, Nowicki TS, Dawson DW, Baranski A, Glaspy JA, Ribas A, Bendall SC, Angelo M

Cell Reports Medicine, October 2021

Multiplexed imaging analysis of the tumor-immune microenvironment reveals predictors of outcome in triple-negative breast cancer

Patwa A, Yamashita R, Long J, Risom T, Angelo M, Keren L, Rubin DL

Communications Biology, July 2021

Multiplexed Ion Beam Imaging Readout of Single-Cell Immunoblotting

Lomeli G, Bosse M, Bendall SC, Angelo M, Herr AE

Analytical Chemistry, June 2021

MAUI (MBI Analysis User Interface)-An image processing pipeline for Multiplexed Mass Based Imaging

Baranski A, Milo I, Greenbaum S, Oliveria JP, Mrdjen D, Angelo M, Keren L

PLoS Computational Biology, April 2021

Single-cell metabolic profiling of human cytotoxic T cells

Hartmann FJ, Mrdjen D, McCaffrey E, Glass DR, Greenwald NF, Bharadwaj A, Khair Z, Verberk SGS, Baranski A, Baskar R, Graf W, Van Valen D, Van den Bossche J, Angelo M, Bendall SC

Nature Biotechnology, August 2020

The Society for Immunotherapy of Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation

Taube JM, Akturk G, Angelo M, Engle EL, Gnjatic S, Greenbaum S, Greenwald NF, Hedvat CV, Hollmann TJ, Juco J, Parra ER, Rebelatto MC, Rimm DL, Rodriguez-Canales J, Schalper KA, Stack EC, Ferreira CS, Korski K, Lako A, Rodig SJ, Schenck E, Steele KE, Surace MJ, Tetzlaff MT, von Loga K, Wistuba II, Bifulco CB, Society for Immunotherapy of Cancer (SITC) Pathology Task Force

Journal for Immunotherapy of Cancer, May 2020

Mapping cell phenotypes in breast cancer

Keren L & Angelo M

Nature Cancer, February 2020

MIBI-TOF: A multiplexed imaging platform relates cellular phenotypes and tissue structure

Keren L, Bosse M, Thompson S, Risom T, Vijayaragavan K, McCaffrey E, Marquez D, Angoshtari R, Greenwald NF, Feinberg H, Wang J, Kambhan N, Kirkwood D, Nolan G, Montine TJ, Galli SJ, West R, Bendall SC, Angelo M

Science Advances, October 2019

Glucose Metabolism Drives Histone Acetylation Landscape Transitions that Dictate Muscle Stem Cell Function

Yucel N, Wang YX, Mai T, Porpiglia E, Lund PJ, Markov G, Garcia BA, Bendall SC, Angelo M, Blau HM

Cell Reports, June 2019

Mass synaptometry: High-dimensional multi parametric assay for single synapses

Gajera CR, Fernandez R, Postupna N, Montine KS, Fox EJ, Tebaykin D, Angelo M, Bendall SC, Keene CD, Montine TJ

Journal of Neuroscience Methods, January 2019

A Structured Tumor-Immune Microenvironment in Triple Negative Breast Cancer Revealed by Multiplexed Ion Beam Imaging

Keren L, Bosse M, Marquez D, Angoshtari R, Jain S, Varma S, Yang S, Kurian A, Van Valen D, West R, Bendall SC, Angelo M

Cell, September 2018

Immunohistochemistry and mass spectrometry for highly multiplexed cellular molecular imaging

Levenson RM, Borowsky AD, Angelo M

Laboratory Investigation, March 2015

Multiplexed ion beam imaging of human breast tumors

Angelo M, Bendall SC, Finck R, Hale MB, Hitzman C, Borowsky AD, Levenson RM, Lowe JB, Liu SD, Zhao S, Natkunam Y, Nolan GP

Nature Medicine, March 2014